基于纳米材料的电化学免疫传感器检测H5N1亚型禽流感病毒的研究

doi:10.11843/j.issn.0366-6964.2013.06.012

Abstract

Abstract:

A novel electrochemical immunosensor for sensitive detection of avian influenza virus (AIV) H5N1 was described. A graphene oxide (GO) carrying H5N1-polychonal antibodies (PAb-H5N1) and Bovine serum albumin (BSA) were used as signal amplification materials. On the basis of the signal amplification strategy of PAb-H5N1-GO-BSA nanocomposite, the developed immunosensor showed a 256-fold increase in detection limit compared to the immunosensor without PAb-H5N1-GO-BSA nanocomposite amplification. The developed method could detect 2^-15 HA unit·50 μL^-1 H5N1 with a linear calibration range from 2^-15 to 2^-8 HA unit·50 μL^-1. This immunosensor has a good specificity and high sensitivity, and it is promising for rapid detection of pathogenic microorganisms.

CLC Number:

HUANG Jiao-ling, XIE Zhi-xun, LUO Si-si, XIE Zhi-qin, XIE Li-ji, LIU Jia-bo, PANG Yao-shan, FAN Qing. A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 911-918.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.06.012

https://www.xmsyxb.com/EN/Y2013/V44/I6/911

References

FOUCHIER R A M, MUNSTER V, WALLENSTEN A, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls［J］. J Virol, 2005, 79(5): 2814-2822.

YUEN K Y, CHAN P K, PEIRIS M, et al. Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus［J］. Lancet, 1998, 351(9101): 467-471.

VELUMANI S, DU Q, FENNER B J, et al. Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens［J］. J Virol Methods, 2008, 147(2): 219-225.

LEE C W, SUAREZ D L. Application of real-time RT-PCR for the quantitation and competitive replication study of H5 and H7 subtype avian influenza virus［J］. J Virol Methods, 2004, 119(2): 151-158.

CHANTRATITA W, SUKASEM C, KAEWPONGSRI S, et al. Qualitative detection of avian influenza A (H5N1) viruses: A comparative evaluation of four real-time nucleic acid amplification methods［J］. Mol Cell Probes, 2008, 22(5-6): 287-293.

李启明, 马学军, 高寒春, 等. 逆转录环介导等温核酸扩增技术(RT-LAMP)在H5N1 AIV基因检测中的应用［J］. 病毒学报, 2008, 24(3): 178-183.

LI D, WANG J, WANG R, et al. A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1［J］. Biosens Bioelectron, 2011, 26(10):4146-4154.

WANG G F, HUANG H, ZHANG G, et al. Dual amplification strategy for the fabrication of highly sensitive interleukin-6 amperometric immunosensor based on poly-dopamine ［J］. Langmuir, 2011, 27(13): 1224-1231.

WU Y, ZHENG J W, LI Z, et al. A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode［J］. Biosens Bioelectron, 2009, 24(5): 1389-1393.

STEFAN R I, BOKRETSION R G, VAN S J F, et al. Immunosensor for the determination of azidothymidine: Its utilization as detector in a sequential injection analysis system［J］. Talanta, 2003, 59(5): 883-887.

WARSINKE A, BENKERT A, SCHELLER F W. Electrochemical immunoassays［J］. Anal Chem, 2000, 366(6-7): 622-634.

HUMMERS W, OFLEMAN R. Preparation of graphite oxide［J］.J Am Chem Soc, 1958, 80:1339-1342.

WANG R, LIN J, LASSITER K, et al. Evaluation study of a portable impedance biosensor for detection of avian influenza virus［J］. J Virol Methods, 2011, 178(1-2): 52-58.

CHARLTON B, CROSSLEY B, HIETALA S. Conventional and future diagnostics for avian influenza［J］. Comp Immunol Microbiol Infect Dis, 2009, 32(4): 341-350.

LUO Q, HUANG H, ZOU W, et al. An indirect sandwich ELISA for the detection of avian influe nza H5 subtype viruses using anti-hemagglutinin protein monoclonal antibody［J］. Vet Microbiol, 2009, 137(1-2): 24-30.

DHUMPA R, HANDBERG K J, JФRGENSEN P H, et al. Rapid detection of avian influenza virus in chicken fecal samples by immunomagnetic capture reverse transcriptase-polymerase chain reaction assay［J］. Diagn Microbiol Infect Dis, 2011, 69(3): 258-265.

SIDOTI F, RIZZO F, COSTA C, et al. Development of real time RT-PCR assays for detection of type A influenza virus and for subtyping of avian H5 and H7 hemagglutinin subtypes［J］. Mol Biotechnol, 2010, 44(1): 41-50.

LAU L T, BANKS J, AHERNE R, et al. Nucleic acid sequence-based amplification methods to detect avian influenza virus［J］. Biochem Biophys Res Commun, 2004, 313(2): 336-342.

WANG J. Nanomaterial-based electrochemical biosensors ［J］. Analyst, 2005, 130(4): 421-426.

LEI J, JU H. Signal amplification using functional nanomaterials for biosensing ［J］. Chem Soc Rev, 2012, 41(6): 2122-2134.

WANG G, ZHANG G, HUANG H, et al. Graphene-Prussian blue/gold nanoparticles based electrochemical immunoassay of carcinoembryonic antigen ［J］. Anal Methods, 2011, 3(9): 2082-2087.

DU D, WANG L, SHAO Y, et al. Functionalized Graphene oxide as a nanocarrier in a multienzyme labeling amplification strategy for ultrasensitive electrochemical immunoassay of Phosphorylated p53(S392) ［J］. Anal Chem, 2011, 83(3): 746-752.

LI Q, ZENG L, WANG J, et al. Magnetic mesoporous organic-inorganic NiCo₂O₄hybrid nanomaterials for electrochemical immunosensors ［J］. ACS Appl Mater Interfaces, 2011, 3(4): 1366-1373.

TANG J, TANG D, NIESSNER R, et al. Magneto-controlled graphene immunosensing platform for simultaneous multiplexed electrochemical immunoassay using distinguishable signal tags ［J］. Anal Chem, 2011, 83(13): 5407-5414.

LIAO Y, YUAN R, CHAI Y, et al. Study on an amperometric immunosensor based on Nafion-cysteine composite membrane for detection of carcinoembryonic antigen ［J］. Anal Biochem, 2010, 402(1): 47-53.

LI J, GAO H, CHEN Z, et al. An electrochemical immunosensor for carcinoembryonic antigen enhanced by self-assembled nanogold coatings on magnetic particles ［J］. Anal Chim Acta, 2010, 665(1): 98-104.

李志英, 许喜林, 刘仲明, 等. 禽流感病毒H9电化学免疫传感器的研制［J］. 生物物理学报, 2011, 27(9): 812-818.

[1]	LI Xin, SUN Yanyan, LIN Mi, CHEN Xiahui, LI Fengsong, BAO Yanfang, YANG Guang, JIANG Tao. Establishment of Colloidal Gold Immunochromatographic Assay for Quantitative Detection of Foot-and-mouth Disease Virus Serotype O, A and Asia 1 [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1042-1052.
[2]	ZHANG Xueting, CHEN Zhengrong, ZHAO Wenwen, HAN Li, CHEN Jianguo. The Establishment of AS-PCR Method to Distinguish Canine Distemper Asia-I Type Wild Strains and Vaccine Strains [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2490-2499.
[3]	XU Lu, XIA Yingju, ZHANG Qianyi, ZHANG Wenwen, WANG Zhen, LI Cui, ZOU Xingqi, ZHU Yuanyuan, XU Yuan, WANG Zhao, ZHAO Qizu, WANG Qin. Analysis of National Proficiency Testing Program for Classical Swine Fever Virus Antibody Detection by ELISA [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2250-2256.
[4]	WANG Junwei, CHEN Fangzhou, KU Xugang, LI Chang, HE Qigai. Establishment and Clinical Application of an Indirect ELISA for Detection of Antibodies against PCV3 Based on Recombinant ORF2 Protein [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 454-460.
[5]	ZHU Jian-bo, YANG Zhen-xing, XIAO Lei, GAO Lin, MIAO Hai-sheng, HE Yu-wen, MENG Jin-xin, YANG Heng, LI Hua-chun. Establishment of a Rapid Competitive ELISA for Detecting Antibodies against Epizootic Haemorrhagic Disease Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1440-1450.
[6]	LUO Shang-xing, FAN Jing-hui, LIU Bao-jing, SHI Qian-kai, HOU Lin-shan, ZUO Yu-zhu. Establishment and Application of the Real-time Reverse Transcription Quantitative PCR Assay for Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 852-858.
[7]	WEI Hou-jun, FAN Zhi-yu, WANG Fang, SONG Yan-hua, HU Bo, QIU Ru-long, CHEN Meng-meng, XU Wei-zhong, XUE Jia-bin. Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 614-619.
[8]	WANG Jia, XIONG Qi-yan, HUA Li-zhong, LIU Bei-bei, GAN Yuan, FENG Zhi-xin, LIU Mao-jun, WU Yu-zi, SHAO Guo-qing. Establishment of an Indirect ELISA Detection Method for Mycoplasma hyorhinis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1932-1938.
[9]	MA Rui，YIN Ren-jie，HUANG Xiao-bo，WEN Xin-tian，YANG Guo-lin，CHANG Xiao-xia，ZHANG Xian，HUA Xiang，ZHAO Yu-jia，CAO San-jie，WEN Yi-ping，WU Rui. Comparision of Two Fluorescence-labeling Methods of Porcine Diarrheal Viruses Detection Microarrays [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 752-761.
[10]	HUA Xiang，HU Zhong-kai，HUANG Xiao-bo，WEN Xin-tian，CAO San-jie，WEN Yi-ping，WU Rui，DENG Jing，ZHAO Song，YIN Ren-jie，CHANG Xiao-xia，OUYANG Da，ZHANG Xian. Construction of cDNA Microarray for Detecting Porcine Epidemic Diarrhea Virus，Porcine Transmissible Gastroenteritis Virus and Porcine Rotavirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2235-2242.
[11]	ZHANG Gai-mei，JIA Hong，HOU Shao-hua，XIN Ting，GUO Xiao-yu，YUAN Wei-feng，LIU Shu-qing，WU Jing，GAO Xin-tao，LI Ming，DONG Rui-kai，ZHU Hong-fei. Establishment of a Double Antibody Sandwich ELISA against Bovine IFN-γ and Its Preliminary Application in Clinical Diagnosis of Bovine Tuberculosis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1693-1698.
[12]	PENG Wu-li， JI Xin-cheng，YU Xue-hui， SHI Qian,WANG Ke-ke， LI Na. A Multiplex PCR Method for Detecting Brucella, Chlamydia psittaci and Coxiella burnetii of Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 123-128.
[13]	WANG Xin, CUI Zhi-zhong, GONG Jian-sen, GAO Ming-yan, FAN Jian-hua,YU Yan, ZHU Jing, XU Bu. Comparison of ELISA and IFA in the Detection of the J Subgroup Avian Leukosis Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1499-1503.
[14]	ZHI Xiao-Ying, REN Wei-Wei, QI Guang-Yu, 吕Jian-Liang,LIANG Zhong, JIANG Tao. Establishment of Judging Standard of Colloidal Gold-based Immunochromatographic Assay Strip for Detection Antibodes of Serotype O Foot-and-mouth Disease [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1415-1421.
[15]	YANG Chun-Hua, ZHOU Yan, SUN Si-Yang, ZHONG Yi, WANG Chuan-Qing. Development and Application of PCR-DHPLC Assays for Detection of the Swine Torque Teno Sus Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1422-1428.

A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments